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Description:Biofilm Tolerance of Antimicrobial Agents Perhaps the most intuitive explanation for why biocides and antibiotics fail to kill microbes in a biofilm is that the antimicrobial agent, here shaded yellow, simply fails to penetrate past the surface layers of the biofilm Figure 1. In this diagram the attachment surface is at the bottom and the fluid in which antimicrobial is delivered is at the top. This video shows the penetration of a red fluorescent dye — rhodamine B — into a staphylococcal biofilm cell cluster Rani et al, The biofilm was grown in the same capillary reactor system described above.

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